IN VITRO CYTOTOXIC ASSESSMENT OF CHITOSAN OLIGOSACCHARIDE LACTATE ON HUMAN BLOOD AND LYMPHOCYTE CELLS

Chitosan oligosaccharides (ChOSs) are the reduced products of chitosan prepared by chemical or enzymatic hydrolysis. The greater solubility and low viscosity ChOSs interest. present study was first to evaluate toxicity oligosaccharide lactate (ChOSlac) in human blood. For this purpose, possible oxidative effects ChOSlac whole blood (hWB) cell viability membrane integrity on lymphocytes (LYMs) were evaluated dose range 10-400 μg/ml for 24 48 hours treatments. Firstly, total antioxidant status (TAS), oxidant (TOS), stress index (OSI) used measure damage hWB serum. Secondly, cytological using 2.3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H tetrazolium-5-carboxanilide inner salt (XTT) dehydrogenase (LDH) assays LYMs. Exposure cells 10-200 doses caused an increase activity a decrease but did not affect cytotoxicity. Conversely, 400 µg/ml relative LDH leakage decreased viability. In summary, has been positively at specific exposure times terms health as contribution its use many areas such being biocompatible, biodegradable, drug carrier molecule.